Brand: VPCIR Enzymes
Monkeypox Virus TOP1 Enzyme
350.00 €+ excl. VAT
High purity, catalytically active monkeypox virus TOP1 Enzyme overexpressed in yeast and purified to single band homogeneity.
| Uses | Research in Cancer, Research in Chronic diseases, Research in Infectious diseases |
|---|---|
| Purity | 80% |
| Sizes | 1.5 µg ≥ 1,500 units, 3.5 µg ≥ 3,500 units, 10 µg ≥ 10,000 units |
| Variants | Single-pack, 2-pack, 3-pack |
| Shipping | Not IATA Restricted |
| Storage | Must be stored at -18°C to -20°C upon arrival |
Monkeypox Virus TOP1 Enzyme (mpvTOP1) is supplied in Dilution Buffer at a minimum concentration of 30ng/µl, corresponding to at least 30 U/µl. 1 U of mpvTOP1 relaxes 0.5 µg of supercoiled pUC19 when incubated in Reaction Buffer and 150 mM NaCl in a total reaction volume of 20 µl at 37 °C for 30 minutes.
The purity and the concentration of the enzyme has been determined by SDS-PAGE followed by Coomassie brilliant-blue staining. The intensity of the mpvTOP1 band has been estimated by densitometry and quantified by using ImageJ software comparing with a protein standard of known concentration.
The mpvTOP1 enzyme is free from nucleases contaminations and 80% pure as determined by SDS-PAGE analysis. The enzyme preparation is highly stable and recommended for activity assays, including cleavage-ligation experiments, equilibrium, nicking and relaxation assays.
The mpvTOP1 enzyme is supplied together with:
• Dilution Buffer: 5 mM Tris-HCl pH 7.5, 400 mM NaCl, 1mM DTT, 50% glycerol.
• Reaction Buffer 10x: 100 mM Tris-HCl pH 7.5; 50 mM MgCl2, 50 mM CaCl2; 50 µg/ml BSA.
• Nucleases-free water.
• 1M NaCl.
Recombinant 6HIS-tagged mpvTOP1 is prepared by overexpressing the enzyme in Saccharomyces cerevisiae followed by purification by affinity chromatography adapted from Knudsen et al, J. Chrom. 1996, DOI: 10.1016/0378-4347(96)00152-1
The enzyme is shipped packed with ice gel packs and must be stored at -18°C to -20°C upon arrival. The temperature must be controlled to avoid that the enzyme preparation solidifies. This would destroy the enzyme integrity and cause a drop of the mpvTOP1 activity. The enzyme has a lifetime of 12 months when stored under optimal conditions.
Certificate of Analysis for Monkeypox Virus Topoisomerase 1 (mpvTOP1).
Safety Data Sheet for Monkeypox Virus TOP1 Enzyme products.
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